Parental exposure to natural mixtures of POPs reduced embryo production and altered gene transcription in zebrafish embryos
Identifieur interne : 000168 ( Main/Exploration ); précédent : 000167; suivant : 000169Parental exposure to natural mixtures of POPs reduced embryo production and altered gene transcription in zebrafish embryos
Auteurs : Jan L. Lyche [Norvège] ; Irena M. Grzes [Pologne] ; Camilla Karlsson [Norvège] ; Rasoul Nourizadeh-Lillabadi [Norvège] ; Vidar Berg [Norvège] ; Anja B. Kristoffersen [Norvège] ; Janneche U. Skare [Norvège] ; Peter Alestr M [Norvège] ; Erik Ropstad [Norvège]Source :
- Aquatic toxicology [ 0166-445X ] ; 2013.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Milieu aquatique.
English descriptors
- KwdEn :
Abstract
Determination of toxicity of complex mixtures has been proposed to be one of the most important challenges for modern toxicology. In this study we performed genome wide transcriptome profiling to assess potential toxicant induced changes in gene regulation in zebrafish embryos following parental exposure to two natural mixtures of persistent organic pollutants (POPs). The mixtures used were extracted from burbot (Lota lota) liver originating from two lakes (Lake Mjøsa and Lake Losna) belonging to the same freshwater system in Norway. The dominating groups of contaminants were polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs) and dichlorodiphenyltrichloroethane metabolites (DDTs). Because both mixtures used in the present study induced similar effects, it is likely that the same toxicants are involved. The Mjøsa mixture contains high levels of PBDEs while this group of pollutants is low in the Losna mixture. However, both mixtures contain substantial concentrations of PCB and DDT suggesting these contaminants as the predominant contributors to the toxicity observed. The observed effects included phenotypic traits, like embryo production and survival, and gene transcription changes corresponding with disease and biological functions such as cancer, reproductive system disease, cardiovascular disease, lipid and protein metabolism, small molecule biochemistry and cell cycle. The changes in gene transcription included genes regulated by HNF4A, insulin, LH, FSH and NF-κB which are known to be central regulators of endocrine signaling, metabolism, metabolic homeostasis, immune functions, cancer development and reproduction. The results suggest that relative low concentrations of the natural mixtures of POPs used in the present study might pose a threat to wild freshwater fish living in the lakes from which the POPs mixtures originated.
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Lyche</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Dept. Food Safety and Infection Biology, Norwegian School of Veterinary Science, POB 8146 Dep.</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Dept. Production Animal Clinical Science, Norwegian School of Veterinary Science, POB 8146 Dep.</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Grzes, Irena M" sort="Grzes, Irena M" uniqKey="Grzes I" first="Irena M." last="Grzes">Irena M. Grzes</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Institute of Environmental Sciences, Jagiellonian University, Gronostajowa 7</s1><s2>30-387 Kraków</s2><s3>POL</s3><sZ>2 aut.</sZ></inist:fA14><country>Pologne</country><wicri:noRegion>30-387 Kraków</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Dept. of Zoology and Ecology, University of Agriculture, Al. A. Mickiewicza 24</s1><s2>31-120 Kraków</s2><s3>POL</s3><sZ>2 aut.</sZ></inist:fA14><country>Pologne</country><wicri:noRegion>31-120 Kraków</wicri:noRegion></affiliation></author><author><name sortKey="Karlsson, Camilla" sort="Karlsson, Camilla" uniqKey="Karlsson C" first="Camilla" last="Karlsson">Camilla Karlsson</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Dept. Production Animal Clinical Science, Norwegian School of Veterinary Science, POB 8146 Dep.</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Nourizadeh Lillabadi, Rasoul" sort="Nourizadeh Lillabadi, Rasoul" uniqKey="Nourizadeh Lillabadi R" first="Rasoul" last="Nourizadeh-Lillabadi">Rasoul Nourizadeh-Lillabadi</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Dept. of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, POB 8146 Dep.</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>4 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Berg, Vidar" sort="Berg, Vidar" uniqKey="Berg V" first="Vidar" last="Berg">Vidar Berg</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Dept. Food Safety and Infection Biology, Norwegian School of Veterinary Science, POB 8146 Dep.</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Kristoffersen, Anja B" sort="Kristoffersen, Anja B" uniqKey="Kristoffersen A" first="Anja B." last="Kristoffersen">Anja B. Kristoffersen</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Norwegian Veterinary Institute, POB 750 Sentrum</s1><s2>0106 Oslo</s2><s3>NOR</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Skare, Janneche U" sort="Skare, Janneche U" uniqKey="Skare J" first="Janneche U." last="Skare">Janneche U. Skare</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Dept. Food Safety and Infection Biology, Norwegian School of Veterinary Science, POB 8146 Dep.</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Norwegian Veterinary Institute, POB 750 Sentrum</s1><s2>0106 Oslo</s2><s3>NOR</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Alestr M, Peter" sort="Alestr M, Peter" uniqKey="Alestr M P" first="Peter" last="Alestr M">Peter Alestr M</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Dept. of Basic Sciences and Aquatic Medicine, Norwegian School of Veterinary Science, POB 8146 Dep.</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>4 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author><author><name sortKey="Ropstad, Erik" sort="Ropstad, Erik" uniqKey="Ropstad E" first="Erik" last="Ropstad">Erik Ropstad</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Dept. Production Animal Clinical Science, Norwegian School of Veterinary Science, POB 8146 Dep.</s1><s2>0033 Oslo</s2><s3>NOR</s3><sZ>1 aut.</sZ><sZ>3 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Norvège</country><placeName><settlement type="city">Oslo</settlement><region nuts="2">Østlandet</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Aquatic toxicology</title><title level="j" type="abbreviated">Aquat. toxicol.</title><idno type="ISSN">0166-445X</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Aquatic toxicology</title><title level="j" type="abbreviated">Aquat. toxicol.</title><idno type="ISSN">0166-445X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aquatic environment</term><term>Brachydanio rerio</term><term>Ecotoxicology</term><term>Embryo</term><term>Persistent organic pollutant</term><term>Toxicity</term><term>Transcription</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Polluant organique persistant</term><term>Embryon</term><term>Transcription</term><term>Milieu aquatique</term><term>Ecotoxicologie</term><term>Toxicité</term><term>Brachydanio rerio</term><term>Danio rerio</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Milieu aquatique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Determination of toxicity of complex mixtures has been proposed to be one of the most important challenges for modern toxicology. In this study we performed genome wide transcriptome profiling to assess potential toxicant induced changes in gene regulation in zebrafish embryos following parental exposure to two natural mixtures of persistent organic pollutants (POPs). The mixtures used were extracted from burbot (Lota lota) liver originating from two lakes (Lake Mjøsa and Lake Losna) belonging to the same freshwater system in Norway. The dominating groups of contaminants were polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs) and dichlorodiphenyltrichloroethane metabolites (DDTs). Because both mixtures used in the present study induced similar effects, it is likely that the same toxicants are involved. The Mjøsa mixture contains high levels of PBDEs while this group of pollutants is low in the Losna mixture. However, both mixtures contain substantial concentrations of PCB and DDT suggesting these contaminants as the predominant contributors to the toxicity observed. The observed effects included phenotypic traits, like embryo production and survival, and gene transcription changes corresponding with disease and biological functions such as cancer, reproductive system disease, cardiovascular disease, lipid and protein metabolism, small molecule biochemistry and cell cycle. The changes in gene transcription included genes regulated by HNF4A, insulin, LH, FSH and NF-κB which are known to be central regulators of endocrine signaling, metabolism, metabolic homeostasis, immune functions, cancer development and reproduction. The results suggest that relative low concentrations of the natural mixtures of POPs used in the present study might pose a threat to wild freshwater fish living in the lakes from which the POPs mixtures originated.</div></front></TEI><affiliations><list><country><li>Norvège</li><li>Pologne</li></country><region><li>Østlandet</li></region><settlement><li>Oslo</li></settlement></list><tree><country name="Norvège"><region name="Østlandet"><name sortKey="Lyche, Jan L" sort="Lyche, Jan L" uniqKey="Lyche J" first="Jan L." last="Lyche">Jan L. Lyche</name></region><name sortKey="Alestr M, Peter" sort="Alestr M, Peter" uniqKey="Alestr M P" first="Peter" last="Alestr M">Peter Alestr M</name><name sortKey="Berg, Vidar" sort="Berg, Vidar" uniqKey="Berg V" first="Vidar" last="Berg">Vidar Berg</name><name sortKey="Karlsson, Camilla" sort="Karlsson, Camilla" uniqKey="Karlsson C" first="Camilla" last="Karlsson">Camilla Karlsson</name><name sortKey="Kristoffersen, Anja B" sort="Kristoffersen, Anja B" uniqKey="Kristoffersen A" first="Anja B." last="Kristoffersen">Anja B. Kristoffersen</name><name sortKey="Lyche, Jan L" sort="Lyche, Jan L" uniqKey="Lyche J" first="Jan L." last="Lyche">Jan L. Lyche</name><name sortKey="Nourizadeh Lillabadi, Rasoul" sort="Nourizadeh Lillabadi, Rasoul" uniqKey="Nourizadeh Lillabadi R" first="Rasoul" last="Nourizadeh-Lillabadi">Rasoul Nourizadeh-Lillabadi</name><name sortKey="Ropstad, Erik" sort="Ropstad, Erik" uniqKey="Ropstad E" first="Erik" last="Ropstad">Erik Ropstad</name><name sortKey="Skare, Janneche U" sort="Skare, Janneche U" uniqKey="Skare J" first="Janneche U." last="Skare">Janneche U. Skare</name><name sortKey="Skare, Janneche U" sort="Skare, Janneche U" uniqKey="Skare J" first="Janneche U." last="Skare">Janneche U. Skare</name></country><country name="Pologne"><noRegion><name sortKey="Grzes, Irena M" sort="Grzes, Irena M" uniqKey="Grzes I" first="Irena M." last="Grzes">Irena M. Grzes</name></noRegion><name sortKey="Grzes, Irena M" sort="Grzes, Irena M" uniqKey="Grzes I" first="Irena M." last="Grzes">Irena M. Grzes</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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